by Rohan Sharma | Feb 16, 2023 | Bioinformatics
As academic researchers, we know that the analysis of next-generation sequencing (NGS) data can be complex and time-consuming. The quality of NGS data directly affects the accuracy of downstream analyses, making it essential to ensure that high-quality data is...
by Rohan Sharma | Sep 27, 2022 | Bioinformatics
One of the pioneering medical device companies specializing in IVF (In Vitro Fertilization) found itself at a crossroads. They already had a multi-billion dollar portfolio of instruments, assays, and culture media and decided to expand their portfolio to include a...
by Rohan Sharma | Sep 12, 2022 | Bioinformatics
Is it necessary to do base quality score recalibration (BQSR) in the GATK pipeline? How should this be done without an available vcf file of known sites? (a) Unfortunately the quality scores produced by next generation sequencing instruments are subject to...
by Rohan Sharma | Aug 27, 2022 | Bioinformatics
Cell and Gene Therapy Biotechnology Company Case Study Time is everything for a bioinformatician. With an active R&D group constantly creating new datasets that need to be analyzed, keeping up with their requests is an uphill battle. The newly...
by Stuart Davis | May 7, 2021 | Bioinformatics
Introduction In the quest to understand the very blueprint of life, DNA sequencing stands as a pivotal technology. With the advent of high-throughput sequencing methods, we’ve entered an era where decoding the genetic material is more accessible than ever. This...
by Stuart Davis | Oct 26, 2019 | Bioinformatics
Trimming for adaptors and low quality bases is important part of the analysis pipeline for sequencing data. Typically, after you isolate and fragment your RNA sample, adaptors are attached to the ends of the sequences that are needed for sequencing (see [1,2] for more...
